Cy7 is a near-IR fluor that is invisible to the naked eye (excitation/emission maximum 750/776nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye. This Cyanine5 NHS ester (analog to Cy5 ® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties. a b Ernst LA, Gupta RK, Mujumdar RB, Waggoner AS (Jan 1989). "Cyanine dye labeling reagents for sulfhydryl groups". Cytometry. 10 (1): 3–10. doi: 10.1002/cyto.990100103. PMID 2917472. Huang, Z. X. et al. Spectral identification of specific photophysics of Cy5 by means of ensemble and single molecule measurements. J. Phys. Chem. A 110, 45–50 (2006). are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic,

The word cyanin is from the English word "cyan", which conventionally means a shade of blue-green (close to "aqua") and is derived from the Greek κυάνεος/ κυανοῦς kyaneos/kyanous which means a somewhat different color: "dark blue". or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST

11Publications Citing Use of MCE Cy5.5

Chemically, cyanines are a conjugated system between two nitrogen atoms; in each resonance structure, exactly one nitrogen atom is oxidized to an iminium. Typically, they form part of a nitrogenous heterocyclic system. [2] Kretschy, N. & Somoza, M.M. Comparison of the sequence-dependent fluorescence of the cyanine dyes Cy3, Cy5, DyLight DY547 and DyLight DY647 on single-stranded DNA. PLoS ONE 9 (2014).

The additive effect of multiple Gs or Cs in a row is not immediately apparent, with GGGGG ranking #31 and #21 (0.71 and 0.64 relative intensity) and CCCCC ranking #1009 and #1021 (0.37 and 0.25 relative intensity) for Cy3 and Cy5, respectively. The top-ranking GAAAA motif identified in 5ʹ-Cy3 oligonucleotides here ranks #334 (0.58 relative fluorescence), further illustrating the significant differences between 5ʹ and 3ʹ labeling. Kim, Eunha; Park, Seung Bum (2010). "Discovery of New Synthetic Dyes: Targeted Synthesis or Combinatorial Approach?". In Demchenko, Alexander P. (ed.). Advanced Fluorescence Reporters in Chemistry and Biology I: Fundamentals and Molecular Design Volume 8 of Springer Series on Fluorescence. Berlin: Springer. p.172. ISBN 9783642047022.

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by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying